An Assay Method of Brain Enzyme Carving β-Endorphin into Methionine Enkephalin
نویسندگان
چکیده
منابع مشابه
Ectopic production of methionine enkephalin and beta-endorphin.
Immunoreactive methionine enkephalin and beta-endorphin were sought by serial dilution of tissue extracts and assay of chromatographic fractions in non-endocrine tumour tissue from three patients with the ectopic adrenocorticotrophin syndrome associated with carcinoid tumours and in normal lung tissue and thymic tissue from a patient with myasthenia gravis. In all cases serial dilution of extra...
متن کاملProteolysis of lipotropin C-fragment takes place extracellularly to form gamma-endorphin and [methionine]enkephalin [proceedings].
The octapeptide 61-68 and the heptapeptide 61-67 do not possess the protected conformation of C-fragment. In contrast with the protracted exposure to membrane enzymes required to form [methioninelenkephalin from C-fragment, the short peptides rapidly undergo this conversion. The corresponding tripeptide Thr-Ser-Glu and dipeptide Thr-Ser were isolated. The results suggest that C-fragment is degr...
متن کاملExpression of substance P, calcitonin gene-related peptide, β-endorphin and methionine-enkephalin in human dental pulp tissue after orthodontic intrusion: a pilot study.
OBJECTIVE To determine the levels of two sensory neuropeptides (substance P [SP] and calcitonin gene-related peptide [CGRP]) and two endogenous opioids (methionine-enkephalin [Met-Enk] and β-endorphin [β-End]) in dental pulp tissue samples subjected to controlled orthodontic intrusive forces. MATERIALS AND METHODS Sixteen healthy premolars were selected from eight patients who were undergoing...
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We summarize here our new discovery that the endogenous opioid peptide β-endorphin (BEP), by virtue of reducing body stress and maintaining active immune system, as well as, balancing the levels of pro-inflammatory and anti-inflammatory cytokines, destroys tumor cells and stops them from being transformed into metastatic cancer cells.
متن کاملQuantitative radioimmunohistochemical method using [125I]-protein A to measure the content of methionine enkephalin in discrete rat brain areas.
We report a quantitative radioimmunohistochemical method, using [125I]-protein A in combination with a specific antibody to methionine enkephalin (Met-enk), for determination of the content of this peptide in discrete areas of rat brain. After paraformaldehyde fixation, rat brain sections were incubated with a Met-enk polyclonal antibody, followed by incubation with [125I]-protein A. After auto...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1978
ISSN: 0021-5198
DOI: 10.1254/jjp.28.930